AN ELECTRON MICROSCOPE STUDY OF DNA MOLECULES FROM CHIRONOMUS TENTANS AND CHIRONOMUS THUMMI
نویسندگان
چکیده
منابع مشابه
An electron microscope study of DNA molecules from Chironomus tentans and Chironomus thummi.
I N recent years substantial information has been gained concerning the size and topology of DNA (deoxyribonucleic acid) molecules of viruses (for reviews see VINOGRAD and LEBOWITZ 1966; COHEN 1967; THOMAS and MACHATTIE 1967) , bacterial plasmic and sex factor (ROTH and HELINSKI 1967; HICKSON, ROTH and HELINSKI 1967), Mycciplasma hominis (BODE and MOROWITZ 1967), bacteria (CAIRNS 1963) and mito...
متن کاملThe Dna Base Composition of Individual Chromosomes and Chromosome Segments from Chironomus Tentans
The base composition of DNA was determined for individual chromosomes from the dipteran Chironomus tentans and for each one of six different segments of one of the chromosomes. The isolations were carried out by micromanipulation and the DNA purines were first extracted from the isolated components and afterwards separated by means of microelectrophoresis on a cellulose fiber. It was found that...
متن کاملDIPTERAN SALIVARY GLAND II. DNase Activity in Chironomus thummi
The deoxyribonuclease (DNase) activity of the dipteran (Chironomus thummi) salivary gland, measured both enzymatically and immunochemically, increases about 7-fold with the onset of metamorphosis. The increase in DNase activity occurs at a time when the activities of other enzymes and the total protein content are decreasing. The increased DNase activity is followed by glandular destruction. It...
متن کاملCloning and expression of an atrazine inducible cytochrome P450, CYP4G33, from Chironomus tentans (Diptera: Chironomidae)
Previous studies performed in our laboratory have measured the effect of atrazine exposure on cytochrome P450-dependent monooxygenase activity and have found increased activity in midge larvae (Chironomus tentans) as a result of atrazine exposure (1– 10 ppm). Here we report the cloning and expression of a specific C. tentans CYP4 gene that is responsive to atrazine induction with an open readin...
متن کاملAtrazine induction of cytochrome P450 in Chironomus tentans larvae.
Cytochrome P450-dependent aldrin epoxidation was characterized in third instar larvae of the aquatic midge, Chironomus tentans. Optimal in vitro assay conditions for the epoxidase were pH 7.6 and 31 degrees C. Activity was linear up to 40 min of incubation time and 0.5 mg microsomal protein per incubation. The activity was concentrated in the microsomal fraction of whole body homogenates and wa...
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ژورنال
عنوان ژورنال: Genetics
سال: 1968
ISSN: 1943-2631
DOI: 10.1093/genetics/60.4.759